Question

Suppose that when an enzyme that breaks RNA chains after each G link is applied to a 12-link chain, the fragments obtained are G, CCG, AAAG, and UCCG, and when an enzyme that breaks RNA chains after each C or U link is applied, the fragments obtained are C, C, C, C, GGU, and GAAAG. Can you determine the entire 12-link RNA chain from these two sets of fragments? If so, what is this RNA chain?

Short answer

The sequence of RNA chain is \(CCGGUCCGAAAG\).

Step-by-step solution

Given that.

Fragment after each\(G\)link applied to\(12 - link\)RNA chains are\(G,CCG,AAAG\).

Fragment after each \(C\) or \(U\)link is applied, are \(C,C,C,C,GGU\).

Concept used.

Method for sequencing RNA chains used enzymes to break chains after links. Some enzymes break RNA chains after each G link while others bread after each C or U link.

Calculation.

Since, the chain breaks after G links therefore the choices are

\(GCCGAAAGUCCG\),\(CCGGAAAGUCCG\)

\(CCGGUCCGAAAG\),\(GAAAGCCGUCCG\),

\(AAAGGCCGUCCG\),\(AAAGCCGGUCCG\),

\(UCCGAAAGGCCG\).

However when the third chain is broken after each U or C, it produces he fragments.

\(C,C,GGU,C,C,GAAAG\).

While the other chains produces he different fragments.

Therefore, the RNA chain is\(CCGGUCCGAAAG\).

Conclusion:

The sequence of RNA chain is \(CCGGUCCGAAAG\).