Chapter 7: Problem 5
The best vector to use to carry a segment of DNA that is \(350 \mathrm{kb}\) in size would be A. bacteriophage. B. bacterial artificial chromosome (BAC). C. cosmid. D. plasmid. E. yeast artificial chromosome (YAC).
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In the selection of bacterial colonies that carry cloned DNA in plasmids, such as \(\mathrm{pBR} 322\), that contain two antibiotic resistance genes, A. one antibiotic resistance gene is nonfunctional in the desired bacterial colonies. B. untransformed bacteria are antibiotic resistant. C. both antibiotic resistance genes are functional in the desired bacterial colonies. D. radiolabeled DNA or RNA probes play a role. E. none of the above.
The X-ray autoradiogram of one strand of a fragment of DNA sequenced by the Sanger method was obtained. The 5 '-cnd of the nucleotide was labeled with \(^{32}\) P. Numbering from bottom to top of the autoradiogram, the lane from the ddG tube showed bands at positions 4 and \(11 ;\) ddC tube had bands at \(1,2,7,8,9,\) and \(13 ;\) ddA at \(5,10,\) and \(15 ;\) and \(\mathrm{dd} \mathrm{d} \mathrm{T}\) at \(3,6,12,\) and \(14 .\) What is the sequence of the fragment? Construct the autoradiogram pattern of the complementary sequence.
Preparation of recombinant DNA requires A. restriction endonucleases that cut in a staggered fashion. B. restriction endonucleases that cleave to yield blunt-ended fragments. C. poly(dT). D. DNA ligase. E. cDNA.
Construction of a restriction map of DNA requires all of the following except A. partial hydrolysis of DNA. B. complete hydrolysis of DNA. C. electrophoretic separation of fragments on a gel. D. staining of an electrophoretic gel to locate DNA. E. cyclic heating and cooling of the reaction mixture.
In the United States, a major cause of death of babies during the first year is sudden infant death syndrome (SIDS). One study showed a strong corrclation for an increased risk of SIDS with a prolonged \(\mathrm{QT}\) interval in their electrocardiograms. In one child, a gene associated with the Long \(\mathrm{QT}\) syndrome had a substitution of AAC for TCC. This gene codes for a protein associated with the sodium channel. The mutation was detected by single-strand conformation polymorphism \((\mathrm{SSCP})\) Which of the following statement(s) about SSCP is/are correct? A. The electrophoretic mobility on polyacrylamide gel of small, single- stranded DNA during the SSCP technique depends partly on the secondary conformation. B. There must be a restriction endonudease site in the region studied for SSCP to work. C. Radiolabeling is not used in this technique. D. It is not necessary to know the sequence of the DNA to be studied. E. All of the above.
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