Chapter 7: Problem 2
Preparation of recombinant DNA requires A. restriction endonucleases that cut in a staggered fashion. B. restriction endonucleases that cleave to yield blunt-ended fragments. C. poly(dT). D. DNA ligase. E. cDNA.
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In the United States, a major cause of death of babies during the first year is sudden infant death syndrome (SIDS). One study showed a strong corrclation for an increased risk of SIDS with a prolonged \(\mathrm{QT}\) interval in their electrocardiograms. In one child, a gene associated with the Long \(\mathrm{QT}\) syndrome had a substitution of AAC for TCC. This gene codes for a protein associated with the sodium channel. The mutation was detected by single-strand conformation polymorphism \((\mathrm{SSCP})\) Which of the following statement(s) about SSCP is/are correct? A. The electrophoretic mobility on polyacrylamide gel of small, single- stranded DNA during the SSCP technique depends partly on the secondary conformation. B. There must be a restriction endonudease site in the region studied for SSCP to work. C. Radiolabeling is not used in this technique. D. It is not necessary to know the sequence of the DNA to be studied. E. All of the above.
Whole genomic DNA was isolated from three individuals, digested separately with a restriction endonuclease to fragments and the fragments separated on agarose gel in an electric field. The gene of interest was isolated and analyzed using Southern blot technique. Each individual sample showed two bands. The bands were identical for two of the individuals. For the third, one band was identical to one band of the other two but the second band was of lower molecular weight than the others. This is an example of restriction fragment length polymorphism (RFLP) The Southern blot technique A. transfers DNA fragments from agarose gel to a nitrocellulose filter. B. requires that the DNA fragments remain double stranded. C. requires that the DNA is radiolabeled prior to addition to the agarose gel. D. alters the position of the DNA fragments during the process. E. amplifies the amount of DNA material.
The best vector to use to carry a segment of DNA that is \(350 \mathrm{kb}\) in size would be A. bacteriophage. B. bacterial artificial chromosome (BAC). C. cosmid. D. plasmid. E. yeast artificial chromosome (YAC).
In the United States, a major cause of death of babies during the first year is sudden infant death syndrome (SIDS). One study showed a strong corrclation for an increased risk of SIDS with a prolonged \(\mathrm{QT}\) interval in their electrocardiograms. In one child, a gene associated with the Long \(\mathrm{QT}\) syndrome had a substitution of AAC for TCC. This gene codes for a protein associated with the sodium channel. The mutation was detected by single-strand conformation polymorphism \((\mathrm{SSCP})\) In order to get enough DNA to analyze, DNA is amplified by a polymerase chain reaction (PCR). In a PCR, A. the nucleotide sequence of the DNA to be amplified must be known. B. the sample is protected from heat which would denature the DNA. C. the role of oligonucleotides in the reaction mixture is to act as primers for the synthesis of new DNA. D. the final product is single-stranded DNA. E. the original DNA can be amplified only about 10 -fold.
What makes the Taq DNA polymerase frequently used for a polymerase chain reaction (PCR) different from other DNA polymerases, and why is it preferable?
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