Chapter 0: Q4P (page 9)
What is the purpose of a calibration curve?
Short Answer
A calibration curve is a graph of detector response vs analyte concentration.
Chapter 0: Q4P (page 9)
What is the purpose of a calibration curve?
A calibration curve is a graph of detector response vs analyte concentration.
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Correcting for matrix effects with an internal standard. The appearance of pharmaceuticals in municipal wastewater (sewage) is an increasing problem that is likely to have adverse effects on our drinking water supply. Sewage is a complex matrix. The drug carbamazepine can be measured at low levels in sewage by liquid chromatography with mass spectral detection. When carbamazepine was spiked into sewage at a concentration of
chromatographic analysis gave an apparent spike recovery of Deuterium (D) is the hydrogen isotope . Deuterated carbamazepine can be used as an internal standard for carbamazepine. The deuterated compound has the same retention time as undertreated material in chromatography, but is distinguished by its higher mass in the mass spectrum. When deuterated carbamazepine was used as an internal standard for the analysis, the apparent recovery was Explain how the internal standard is used in this analysis and rationalize why it works so well to correct for matrix effects.
The relative retention for two compounds in gas chromatography is 1.068 on a column with a plate height of 0.520mm.The retention factor for compound 1 is 5.16.
(a) Find the retention factor for compound 2.
(b) What length of column will separate the compounds with a resolution of 1.50 ?
(c) The retention time for air () is 2.00 min. If the number of plates is the same for both compounds, Findfor each peak.
(d) If the volume ratio of stationary phase to mobile phase is 0.30,Find the partition coefficient for compound 1.
Effect of pH on the EDTA titration. Use Equation 12-11 to compute curves (pCa2+ versus mL of EDTA added) for the titration of 10.00 mL of 1.00 mM Ca2+ with 1.00 mM EDTA at pH 5.00, 6.00, 7.00, 8.00, and 9.00. Plot all curves on one graph and compare your results with Figure 12-10.
Titration of EDTA with metal ion. Use Equation 12-12 to reproduce the results of Exercise 12-C
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