Question

Why is it advantageous to use large particles \(\left( {{\bf{50}}{\rm{ }}\mu {\bf{m}}} \right)\) for solid phase extraction, but small particles \(\left( {{\bf{5}}{\rm{ }}\mu {\bf{m}}} \right)\) for chromatography?

Short answer

• If you're working with an aqueous base sample and want to inject it into a GC instrument to convert it to a volatile organic, solid phase extraction could help you achieve your aim.
• Chromatography helps in purification,precise separation and analysis. It necessitates very small sample volumes.

Step-by-step solution

Definition ofChromatography.

• Chromatography is a fashion for separating the factors, or solutes, of a mixture grounded on the relative quantities of each solute distributed between a flowing fluid sluice, known as the mobile phase, and a stationary phase that's conterminous.

Determine the advantages for solid particles and small particles for chromatography.

Solid phase extraction:

• As per the physical and chemical properties the solid phase extraction helps in separating compounds dissolved or suspended in a liquid solution from other compounds in the mixture
• Without application of high-pressure large particles can be allowed for the sample to drain through the column.

Chromatography

• The separation of mixtures whilst using a mobile phase (moving solvent) on stationary phase\( \to \)mostly due to different polarity of each compound in the mixture.
• Small particles - cause an increase in the efficiency of the separation. Using of these molecules requirehigh-pressure application in order to "push" the solvent through the column.
• It can analyse pharmaceuticals, food particles, plastics, pesticides, air and water samples, and tissue extracts, among other things.