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Blind Samples: Interpreting Statistics-- The U.S. Department of Agriculture provided homogenized beef baby food samples to three labs for analysis.4Results from the labs agreed well for protein, fat, zinc, riboflavin, and palmitic acid. Results for iron were questionable: Lab A, 1.59±0.14(13); Lab B, 1.65±0.56 (8); Lab C, 2.68±0.78(3) mg/100 g . Uncertainty is the standard deviation, with the number of replicate analyses in parentheses. Use two separate t tests to compare results from Lab Cwith those from Lab A and Lab B at the 95 % confidence level. Comment on the sensibility of the t test results and offer your own conclusions.

Short Answer

Expert verified

According to the t test results, Lab C is substantially greater than Lab B but not significantly greater than Lab A. These results don't add up, so it's proposed that we increase the number of trials/replicates in Lab C.

Step by step solution

01

Definition of F test and t test

  • The F-test is used to compare standard deviations. When comparing one sample to another, or a sample to what we would expect to find given a certain population distribution, we want to determine if the spread or dispersion of the two sets of data is comparable.
  • A t-test is a statistical test used to compare the means of two groups that are related in some way.
02

Determine the F test for lab C and lab A, the t test for lab C and lab A

To utilize distinct t tests to compare the results from Lab C with those from Lab A and Lab B, we must first conduct an F test in each of them to determine which specific case of t test to apply.

(i) Lab C and Lab A

F test

Fcalc=s12s22=0.7820.142=31

Ftable= 3.88 (based on Table 4 - 3 degrees of freedom for: s1=2ands2=12)

Since Fcalc>Ftableat the 95 % confidence level, there is a considerable discrepancy between the variances and standard deviations of the two lab values.

(i) Lab C and Lab A

t test

Find the case 2 b since it is comparing two findings with standard deviations that are significantly different, i.e., role="math" localid="1663561377979" Fcalc>Ftable-

tcalc=x1-x2s12/n1+s22/n2=2.68-1.590.7823+0.14213=2.41

ttable=4.303(based on Table 4-4; degrees of freedom -2; 95 % confidence level)

Since tcalc<ttable at the 95 %confidence level, there is no significant difference between the means of the two lab reports.

03

Determine the F test for lab C and lab B, the t test for lab C and lab B

(ii) Lab C and Lab B

F test

Fcalc=s12s22=0.7820.562=1.94

Ftable=4.74 (based on Table 4-3; degrees of freedom for: s1=2and s2=7)

Since Fcalc<Ftable at the 95 %confidence level, there is no significant difference between the variances and standard deviations of the two lab values.

(ii) Lab C and Lab B

t test

Because it will compare two findings with standard deviations that aren't considerably different and select Case 2 a., i.e., Fcalc<Ftable

First, start by calculating the spooledas a precondition for solving tcalc-

Spoonled=s12(n1-1)+s22(n2-1)n1+n2-nt=0.782(3-1)+0.562(8-1)3+8-2=0.6157199941

04

Determine the tcalc and contrast it to ttable .

Now, calculate for tcalcand contrast it to ttable.

tcalc=x1-x2Spooledn1n2n1+n2=2.68-1.650.6157199941383+8=2.47

ttable=2.262 (Degrees of freedom are based on Table 4-4. localid="1663562198639" n1+n2-nt=3+8-2=9; 95% level of assurance)

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Most popular questions from this chapter

Standard addition graph. Allicin is a ~0.4wt%component in garlic with antimicrobial and possibly anticancer and antioxidant activity. It is unstable and therefore difficult to measure. An assay was developed in which the stable precursor alliin is added to freshly crushed garlic and converted to allicin by the enzyme alliinase found in garlic. Components of the garlic are extracted and measured by chromatography. The chromatogram shows standard additions reported as mg alliin added per gram of garlic. The chromatographic peak is allicin from the conversion of alliin.

(a)The standard addition procedure has a constant total volume. Measure the responses in the figure and prepare a graph to find how much alliin equivalent was in the unspiked garlic. The units of your answer will be mg alliin/g garlic. Find the 95%confidence interval, as well.

(b) Given that2molof alliin are converted to1molof allicin, find the allicin content of garlic (mg allicin/g garlic) including the95%confidence interval.

Standard addition. An unknown sample of Ni2+gave a current of 2.36μAin an electrochemical analysis. When 0.500mLof solution containing role="math" localid="1654761474124" 0.0187MNi2+was added to 25.0mLof unknown, the current increased to 3.79μA.

(a) Denoting the initial, unknown concentration as [Ni2+], write an expression for the final concentration, [Ni2+]f, after role="math" 25.0mLof unknown were mixed with 0.500mLof standard. Use the dilution factor for this calculation.

(b) In a similar manner, write the final concentration of added standard Ni2+, designated as [S]f.

(c) Find[Ni2+]in the unknown.

Why is it desirable in the method of standard addition to add a small volume of concentrated standard rather than a large volume of dilute standard?

Internal standard. A solution was prepared by mixing 5.00mLof unknown elementXwith2.00mLof solution containingrole="math" localid="1654777035083" 4.13μgof standard elementSper millilitre, and diluting to10.0mL. The signal ratio in atomic absorption spectrometry was (signal fromX)/ (signal fromS)=0.808. In a separate experiment, with equal concentrations ofXandS, (signal fromX)/signal fromS)=1.31. Find the concentration ofXin the unknown.

Olympic athletes are tested to see if they are using illegal performance-enhancing drugs. Suppose that urine samples are taken and analyzed and the rate of false positive results is 1 %. Suppose also that it is too expensive to refine the method to reduce the rate of false positive results. We do not want to accuse innocent people of using illegal drugs. What can you do to reduce the rate of false accusations even though the test always has a false positive rate of 1 %?

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