Question

35: What are the differences between a reconstructed total ion chromatogram, an extracted ion chromatogram, and a selected ion chromatogram?

Short answer

Select ion chromatograms and extract ion chromatograms were both recorded more selectively compared to other monitoring method.

Step-by-step solution

 Chromatograms

Chromatograms are graphs of detector response versus time. In the case of mass spectrometry, the detector response is usually in the form of ion current readings.

 Answering the above mentioned question

Chromatograms are graphs of detector response versus time. In the case of mass spectrometry, the detector response is usually in the form of ion current readings.

Reconstructed total ion chromatograms are composed of multiple mass spectra readings that were ‘‘reconstructed‘‘ to form one chromatogram. The readings were done for all observable m/z values.

Select ion chromatograms, however, are ion current readings with a maximum of five m/z values’ peaks. Only readings from these m/z values are detected and presented in chromatogram.

Select ion chromatograms and extract ion chromatograms were both recorded more selectively compared to other monitoring method.

Extracted ion chromatograms are similar to select ion chromatograms because they both only show current