Chapter 23: Q40P (page 631)
Explain how slinization reduces tailing of chromatographic peaks.
Short Answer
Silanization creates hydroxyl groups that can form strong hydrogen bonds.
Chapter 23: Q40P (page 631)
Explain how slinization reduces tailing of chromatographic peaks.
Silanization creates hydroxyl groups that can form strong hydrogen bonds.
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Get started for freeA Gas chromatogram of a mixture of toluene and ethyl acetate is shown here:
(a)Use the width of each peak (measured at the base)to calculate the number of theoretical plates in the column. Estimate all lengths to the nearest 0.1 mm.
(b)Using the width of the toluene peak at its base, calculate the width expected at half height. Compare the measured and calculated values. When the thickness of the the line is significant relative to the length being measured, it is important to take the pen line width into account. You can measure from the edge of one line to the corresponding edge of the other line,as shown here.
An open tubular column has an inner diameter of and the thickness of the stationary phase on the inner wall is . Unretained solute passes through in 63s and a particular solute emerges in 433s . Find the partition coefficient for this solute and find the fraction of time spent in the stationary phase.
A chromatogram with ideal Gaussian bands hasand
.
(a) How many theoretical plates are present?
(b) Find the plate height if the column is 10cm long.
(a) A chromatography column with a length of 10.3 cmand inner diameter of 4.61mmis packed with a stationary phase that occupies 61.0% of the volume. If the volume flow rate is 1.13mL, find the linear velocity in cm/min.(b) How long does it take for solvent (which is the same as unretained solute) to pass through the column? (c) Find the retention time for a solute with a retention factor of 10.
23-41. Describe how nonlinear partition isotherms lead to non-Gaussian band shapes. Draw the chromatographic peak shapes produced by an overloaded gas chromatography column and an overloaded liquid chromatography column.
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