Question

Consider a chromatography column in which${\mathit{V}}_{\mathbf{s}}\mathbf{=}{\mathit{V}}_{\mathbf{m}}\mathbf{/}\mathbf{5}$ Find the retention factor if K = 3 and if K = 30 .

Short answer

a) Retention factor when $K=3$ is 0.6

b) Retention factor when is$K=30$ is 6 .

Step-by-step solution

Given Information:

$$\begin{gathered} V_s=V_m/5 \\ K=3, \\ K=30 \end{gathered}$$

To find the Retention factor when K = 3 :

$V_S/V_m=1/5$

Retention factor is given by;

$$\begin{gathered} k=K\times \frac{V_s}{V_m} \\ k=3 \\ K=3\times \frac{1}{5} \\ K=\frac{3}{5}=0.6 \end{gathered}$$

To find the Retention factor when K = 30 :

$$\begin{gathered} K=30 \\ k=K\times \frac{V_s}{V_m} \\ k=30\times \frac{1}{5} \\ k=\frac{30}{5} \\ k=6 \end{gathered}$$