Question

(a) List ways in which the resolution between two closely spaced peaks might be changed.

(b) After optimization of an isocratic elution with several solvents, the resolution of two peaks is 1.2How might you improve the resolution without changing solvents or the kind of stationary phase?

Short answer

a) The resolution between two closely spaced peaks might be changed

- by changing the fraction of each solvent

- by changing the solvent

- by changing the PH

- by changing the stationary phase

- by changing the temperature

Step-by-step solution

Resolution of elution definition

The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation.

Improving the resolution  

a) The resolution between two closely spaced peaks might be changed

- by changing the fraction of each solvent

- by changing the solvent

- by changing the PH

- by changing the stationary phase

- by changing the temperature

Question: (b) After optimization of an isocratic elution with several solvents, the resolution of two peaks is How might you improve the resolution without changing solvents or the kind of stationary phase?

Answer:

To improve the resolution without changing solvents or the kind of stationary phase we can use a different temperature, longer column, slower flow rate and smaller particle size.

Resolution of elution definition

The resolution of a elution is a quantitative measure of how well two elution peaks can be differentiated in a chromatographic separation.

Improving the Resolution  

To improve the resolution without changing solvents or the kind of stationary phase we can use a different temperature, longer column, slower flow rate and smaller particle size