Question

What are the general steps in developing as isocratic separation for reversed-phase chromatography with one organic solvent and temperature as variable?

Short answer

General steps in developing as isocratic separation.

To develop an isocratic separation for reversed-phase chromatography with one organic solvent .

Step-by-step solution

definition of general steps

To develop an isocratic separation for reversed-phase chromatography with one organic solvent.

And temperature as variables we need to change %B and temperature (T) using this steps

following this steps

in run A$\to$high %B and low T

in rub B$\to$high %B and high T

in rub C $\to$low %B and high T

in rub D $\to$low %B and low T

Using the chromatograms, determine the conditions between runs A, B, C and D.

Hence,

To develop an isocratic separation for reversed-phase chromatography with one organic solvent and temperature as variables we need to change %B and temperature (T) using this steps

in run A$\to$high %B and low T

in rub B$\to$high %B and high T

in rub C$\to$low %B and high T

in rub D$\to$low %B and low T

Using the chromatograms, determine the conditions between runs A, B, C and D.