Question

Question: what are the general steps in developing an isocratic separation for reversed-phase chromatography?

Short answer

General steps in developing an isocratic separation.

• determine the analysis's goal.
• choose a sample preparation method.

Choose a detector that runs a wide gradient to determine whether isocratic

Step-by-step solution

definition an isocratic separation

To create an isocratic separation for reversed-phase chromatography, we need to follow this steps.

• Determine the analysis's goal.
• choose a sample preparation method.
• Choose a detector that runs a wide gradient to determine whether isocratic or gradient elution is appropriate.

continue to follow this step

Then,

• for isocratic elution adjust the mobile phase composition to achieve retention of$0.5\le \mathrm{k}\le 20$for all compounds.
• Adjust relative retention by varying the solvent composition, column temperature, organic solvent type, and column type.
• optimise column dimensions in order to increase plate number or decrease separation time.

Hence,.

• Determine the analysis's goal. choose a sample preparation method. Choose a detector that runs a wide gradient to determine whether isocratic or gradient elution is appropriate. for isocratic elution adjust the mobile phase composition to achieve retention of $0.5\le \mathrm{k}\le 20$for all compounds. Adjust relative retention by varying the solvent composition, column temperature, organic solvent type, and column type. optimise column dimensions in order to increase plate number or decrease separation time.