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Chapter 25: Q29P (page 710)

What does it mean for a separation procedure to be ”rugged” and why is it desirable?

Short Answer

Expert verified

A separation procedure to be rugged and why is it desirable.

It is desirable because that small changed conditions do not change the final outcome of separation.

Step by step solution

01

definition of rugged

characterised by a pragmatic, unemotional attitude or point of view tenacious; unyielding.

When the separation procedure is "rugged," it is not affected by pH, temperature, gradual deterioration of the column, or minor changes in solvent composition.

It is desirable because minor changes in conditions have no effect on the final outcome of separation

02

Why is it desirable

It is desirable because that small changed conditions do not change the final outcome of separation.

Hence,

When the separation procedure is "rugged," it is not affected by pH, temperature, gradual deterioration of the column, or minor changes in solvent composition. It is desirable because that smallchanged conditions do not change the final outcome of separation.

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Most popular questions from this chapter

HPLC peak should generally not have an asymmetry factor, B/A in figure $23 - 14$ ,outside the range $0.9 - 1.5$

Sketch the shape of a peak with an asymmetry of 1.8
What might you do to correct the asymmetry?

(a) You wish to use a wide gradient from 5 vol % to 95 vol %B for the first separation of a mixture of small molecules to decide whether to use gradient or isocratic elution. What should be the gradient time, $t_{G}$ ,for a $15 \times 0.46 - cm$ column containing $3 - μm$ particles with a flow of $1.0 mL / \min$

You optimized the gradient separation going from 20vol%to 34 vol% Bin 11.5min at 1.0mLFind $k *$ for this optimized

separation. To scale up to a $15 \times 1.0 cm$ column, what should be the gradient time and the volume flow rate? If the sample load on

the small column was 1mgwhat sample load can be applied to the large column? Verify that $k *$ is unchanged.

use figure $25 - 17$ to suggest which type of liquid chromatography you could use to separate compounds in each of the following categories.

(a)Molecular mass <2000,soluble in octane

(b) Molecular mass <2000 ,soluble in methanol-water mixtures

(c) Molecular mass <2000 ,weak acid

(d)Molecular mass<2000 ,soluble highly polar

(e) Molecular mass <2000 ,ionic

(f)Molecular mass<2000,soluble in water in nonionic various

(g) Molecular mass<2000,soluble in water in water, variety of changes

(h) Molecular mass<2000,soluble in tetrahydrofuran

Question:The graph shows retention data from a $C_{8}$ silica column with an acetonitrile/water mobile phase.

(a) What mobile phase composition provides greatest retention $(k)$ for the components? Least retention? Coelution $(equal k)$ of two components?

(b) Predict the retention time of each peak at $40 % and 60 % acetonitrite$ . Draw a chromatogram (a "stick diagram" representing each peak as a vertical line) of the separation at each mobile phase composition.

(c) Would $60 %$ acetonitrile yield adequate resolution?

(d) Assuming Gaussian peaks, does the separation at $60 %$ acetonitrile have the attributes of a good separation?

(a) Why does mobile phase strength increase as solvent

becomes less polar in reversed-phase chromatography, whereas

mobile phase strength increases as solvent becomes more polar in

normal-phase chromatography?

(b) What kind of gradient is used in supercritical fluid chromatography?

See all solutions

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