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(a) How can you improve the resolution between two closely spaced peaks in gas chromatography?

(b) What approach from (a) would be most cost effective (not involve a purchase)?

Short Answer

Expert verified

(a). In gas chromatography, to show how to improve the resolution between two closely spaced peaks.

(b). Optimizing the flow rate and temperature in gas chromatography is the most effective way to improve the resolution between the two closely spaced peaks.

Step by step solution

01

Definition of chromatography 

As a result of differential distribution of the solutes as they flow over or over a stationary liquid or solid phase, a chemical mixture transported by a liquid or gas is split into components.

02

Determine the resolution between two closely spaced peaks in gas chromatography  

(a)

  • It is necessary to discuss how to improve the resolution between two closely spaced peaks in gas chromatography.
  • In gas chromatography, to show how to improve the resolution between two closely spaced peaks.
  • In gas chromatography, there are several approaches to increase the resolution between two closely spaced peaks:
  • Increase the column's length while decreasing its diameter.
  • By altering the stationary phase of the system.
  • By maximising the pace of flow.
  • Increase the retention factor by increasing the thickness of the film as the temperature drops.
03

Determine in what approach from (a) would be most cost effective (not involve a purchase)

(b)

  • It is necessary to take the most cost-effective technique.
  • To provide the most cost-effective solution
  • Optimizing the flow rate and temperature in gas chromatography is the most effective way to improve the resolution between the two closely spaced peaks.

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Most popular questions from this chapter

Efficiency of solid-phase microextraction. Equation24-9gives the mass of analyte extracted into a solid-phase microextraction fiber as a function of the partition coefficient between the fiber coating and the solution.

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(b) Evaluate the limit of Equation24-9asVr gets big relative to KVf. Does the extracted mass in your graph approach this limit?

(c) What percentage of the analyte fromof solution is extracted into the fiber when and whenK=100andwhenk=10000?

(a) When a solution containing234mg of pentanol (FM 88.15) and237mg of 2,3 -dimethyl-2-butanol (FM 102.17) in10.0ml was separated, relative peak areas were pentanol: 2,3 -dimethyl-2-butanol = 0.913 : 1.00. Considering pentanol to be the internal standard, find the response factor for 2,3 -dimethyl-2-butanol.

(b) Use Equation 24-8 to find the areas for pentanol and 2,3 -dimethyl-2-butanol in Figure 24-8.

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(a) What types of solutes are typically separated with a poly(dimethylsiloxane)-coated open tubular column?

(b) What types of solutes are typically separated with a poly(ethylene glycol)-coated open tubular column?

(c) What types of solutes are typically separated with a porous-layer open tubular column?

Consider the chromatography of n-c12H26on a 25 m ร— 0.53 mm open tubular column of 5% phenylโ€“95% methyl polysiloxane with a stationary phase thickness of 3.0 ฮผmand He carrier gas at 125ยบC. The observed retention factor for n-c12H26is 8.0. Measurements were made of plate height, H, at various values of linear velocity,ฮผxm/s. A least-squares curve through the data points is given by

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This problem reviews concepts from Chapter 23. An unretained solute passes through a chromatography column in 3.7 min and analyte requires 8.4 min.

(a) Find the adjusted retention time and retention factor for the analyte.

(b) Find the phase ratio b for a 0.32-mm-diameter column with a 1.0-mm-thick film of stationary phase.

(c) Find the partition coefficient for the analyte.

(d) Determine the retention time on a similar length of 0.32-mm diameter column with a 0.5-mm-thick film of the same stationary phase at the same temperature.

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