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Chapter 24: Q11P (page 663)

What is the purpose of derivatization in chromatography? Give an example.

Short Answer

Expert verified

Non-volatile carboxylic acid is converted to volatile trimethylsilyl ester derivative in the process of derivatization.

Step by step solution

01

Meaning of purpose of derivatization in chromatography

Derivatization in gas chromatography is a sample preparation technique that is performed to analytes for Converting them into forms that are easy to detectAn example of derivatization is the conversion of non-volatile carboxylic acid into volatile trimethylsilyl ester derivative, as shown below.

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Most popular questions from this chapter


In the analysis of odorants in tequila in Figure 24-21, tequila was diluted with water and extracted four times with dichloromethane(CH2Cl2) , b.p. 40โˆ˜CThe400mLof(CH2Cl2)was evaporated down to1mLand1ฮผLof the extract was injected on-column onto a poly(ethylene glycol) open tubular column (30mร—0.53mm,filmthickness=1ฮผm), film thickness ) initially at and then ramped to 230โˆ˜C

(a) Why was the diluted tequila extracted four times with dichloromethane instead of once with a larger volume?

(b) Why was on-column injection used?

(c) Why was a poly(ethylene glycol) column chosen for this application?

(d) What was the phase ratio of the column?

(e) Why was a wide-bore 0.53-mm-diameter column chosen for this application?

3. (a) What are the advantages and disadvantages of using a narrower open tubular column?

(b) What are the advantages and disadvantages of using a longer open tubular column?

(c) What are the advantages and disadvantages of using a thicker film of stationary phase?

(a) When a solution containing234mg of pentanol (FM 88.15) and237mg of 2,3 -dimethyl-2-butanol (FM 102.17) in10.0ml was separated, relative peak areas were pentanol: 2,3 -dimethyl-2-butanol = 0.913 : 1.00. Considering pentanol to be the internal standard, find the response factor for 2,3 -dimethyl-2-butanol.

(b) Use Equation 24-8 to find the areas for pentanol and 2,3 -dimethyl-2-butanol in Figure 24-8.

(c) The concentration of pentanol internal standard in the unknown solution was93.7mM . What was the concentration of 2,3 -dimethyl2-butanol?

(a) If retention times are 1.0 min for CH4, 12.0 min for octane, 13.0 min for unknown, and 15.0 min for nonane, find the Kovats retention index for the unknown. (b) What would be the Kovats index for the unknown if the phase ratio of the column were doubled? (c) What would be the Kovats index for the unknown if the length of the column were halved

This problem reviews concepts from Chapter 23 using

Figure 24-7.

(a) Calculate the number of theoretical plates (N in Equation 23-30)

and the plate height (H) for CO.

(b) Find the resolution (Equation 23-23) between argon and oxygen.
See all solutions

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