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Chapter 18: Q33P (page 459)

What is the difference between luminescence and Chemi-luminescence?

Short Answer

Expert verified

Chemi-luminescence is a type of luminescence that occurs during chemical reactions in some substances.

Luminescence is the emission of electromagnetic radiation (mostly light, but also ultraviolet and infrared radiation) which is not excited by a thermal process and elevated temperature of the substance but is due to receiving energy in some other form.

Step by step solution

01

Chemi-luminescence:

Chemiluminescence is the release of light from a chemical reaction.

02

Luminescence:

Luminosity is the sudden release of light by a substance not produced by heat; Or "cold light". It is therefore a form of cold body radiation.

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Most popular questions from this chapter

Calculate the frequency (Hz), wavelength $({cm}^{- 1})$ , and energy (J/photon and J/mols of photon]) of visible light with a wavelength of 562 nm.

Ammonia can be determined spectrophotometrically by reaction with phenol in the presence of hypochlorite $({OCl}^{-})$

A 4.37 - mgsample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0mL. Thenof the solution were placed in a 50 - mLvolumetric flask and treated with 5mLof phenol solution plus 2mLof sodium hypochlorite solution. The sample was diluted to 50.0mLand the absorbance at 625nmwas measured in a 1.00 - cmcuvet after 30min. For reference, a standard solution was prepared from 0.0100g of ${NH}_{4} Cl$ dissolved in 1.00Lof water. Then 10.0mLof this standard were placed in a50 - mL volumetric flask and analyzed in the same manner as the unknown. A reagent blank was prepared by using distilled water in place of unknown.

(a)Calculate the molar absorptivity of the blue product.

(b)Calculate the weight percent of nitrogen in the protein.

18-6. Box 3-1 showed a single-beam spectrophotometric ozone monitor. The emission spectrum of the mercury vapor lamp is shown in Box 18-2.

What is the light source?
What is the detector?
What is the monochromator?
What are the components of the optical pathlength, b?
How does this instrument measure O3?

18-27. The iron-binding site of transferrin in Figure 18-8 can accommodate certain other metal ions besides Fe and certain other anions besidesData are given in the table for the titration of transferrin ( 3.57 mg in 2.00 mL ) with 6.64 mM ${Ga}^{3 +}$ solution in the presence of the anion oxalate, $C_{2} O_{4}^{2 -}$ , and in the absence of a suitable anion. Prepare a graph similar to Figure 18-11, showing both sets of data. Indicate the theoretical equivalence point for the binding of one and two ${Ga}^{3 +}$ ions per molecule of protein and the observed end point. How many ${Ga}^{3 +}$ ions are bound to transferrin in the presence and in the absence of oxalate?

Preparing standards for a calibration curve.

(a) How much ferrous ethylene diammonium sulfate $(Fe (H_{3} {NCH}_{2} {CH}_{2} {NH}_{3}) {({SO}_{4})}_{2} \cdot 4 H_{2} O, FM 382 .15)$ should be dissolved in a 500mL volumetric flask with $1 {MH}_{2} {SO}_{4}$ to obtain a stock solution with $~ 500 μ gFe / {mL}^{2}$ ?

(b) When making stock solution (a), you actually weighed out 1.627 g of reagent. What is the Fe concentration in role="math" localid="1668357579931" $500 μ gFe / {mL}^{2}$ ?

(c) How would you prepare 500 mL of standard containing 1,2,3,4, and $5 μ gFe / {mL}^{2} in 0 .1 {MH}_{2} {SO}_{4}$ infrom stock solution (b) using any Class A pipets from Table 2-4 with only 500 -mL volumetric flasks?

(d) To reduce the generation of chemical waste, describe how you could prepare 50 mL of standard containing, andinfrom stock solution (b) by serial dilution using any Class A pipets from Table 2-3 with only 50mL volumetric flasks?

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