Chapter 26: Q25P (page 748)
In the separation of proteins by hydrophobic interaction chromatography, why does eluent strength increase with decreasing salt concentration in the aqueous eluent?
Decreasing salt concentration increases protein solubility in aqueous mobile phase.
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(a) A particular solution in a particular capillary has an electroosmotic mobility of and 8.1 . How long will it take a neutral solute to travel 52 cm from the injector to the detector if 27 kV is applied across the 62-cm-long capillary tube at pH 2? At pH 12?(b) An analyte anion has an electrophoretic mobility of How long will it take to reach the detector at pH 2?At pH 12?
A gel filtration column has a radius, y.of 0.80 cm and a length,l, of 20.0 cm
(a).Calculate the volume,of the column, which is equal to
(b). The interstitial volumewas 18.1 mL and the total volume of mobile phase was 35.8 mL.
Find.for a solute eluted at 27.4 mL.
(a) The interstitial volume, , in Figure 26-15 is the volume at which the curves rise vertically at the left. Findfor the 25-nm-pore-size column. To the nearest order of magnitude, what is the smallest molecular mass of molecules excluded from this column? (b) What is the molecular mass of molecules eluted at 9.7 mL from the 12.5-nm column? (c)is the volume at which the curves drop vertically at the right. Find the largest molecular mass that can freely enter the 45-nm pores.
Figure 26-24 shows the effect on resolution of increasing voltage from 28 to120kV.
(a) What is the expected ratio of migration times in the two experiments? Measure the migration times for peak 1 and find the observed ratio.
(b) What is the expected ratio of plates in the two experiments?
(c) What is the expected ratio of bandwidths?
(d) What is the physical reason why increasing voltage decreases bandwidth and increases resolution?
Explain how neutral molecules can be separated by micellar electrokinetic chromatography. Why is this a form of chromatography? Why are micelles called a pseudostationary phase?
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