Chapter 26: Q1P (page 746)
State the effects of increasing cross-linking on an ion-exchange column.
Effects of increasing cross-linking on an ion-exchange column will be explained.
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26-D. Consider a capillary electrophoresis experiment conducted near pH9, at which the electroosmotic flow is stronger than the electrophoretic flow.
(a) Draw a picture of the capillary, showing the placement of the anode, cathode, injector, and detector. Show the direction of electroosmotic flow and the direction of electrophoretic flow of a cation and an anion. Show the direction of net flow.
(b) Using Table 15-1, explain why has a shorter migration time than Predict whether will have a shorter migration time than or a greater migration time than
(c) Why is the mobility of greater than that of?
What is the principal source of zone broadening in ideal capillary electrophoresis?
(a) Suppose that the reservoir in Figure 26-7 contains 1.5 L of 2.0 M . For how many hours can the reservoir provide 20mM KOH at a flow rate of 1.0 mL/min if 75% consumption ofin the reservoir is feasible?
(b) What starting and ending current would be required to produce a gradient from 5.0 mM KOH to 0.10 M KOH at 1.0 mL/min flow rate?
What is electroosmosis?
The water-soluble vitamins niacinamide (a neutral com- pound), riboflavin (a neutral compound), niacin (an anion), and thiamine (a cation) were separated by micellar electrokinetic chromatography in 15mMborate buffer (pH8.0)with 50mMsodium dodecyl sulphate. The migration times were niacinamide (8.1min), riboflavin (13.0min), niacin (14.3min), and thiamine. What would the order have been in the absence of sodium dodecyl sulphate? Which compound is most soluble in the micelles?
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