Question

(a) How can molecular exclusion chromatography be used to measure the molecular mass of a protein?

(b) Which pore size in Figure 26-15 is most suitable for chromatography of molecules with molecular mass near 100 00?

Short answer

The solutions to the above questions are:

1. Molecular exclusion chromatography
2. 25 nm

Step-by-step solution

Molecular exclusion chromatography:

1. Molecular exclusion chromatography – also called Gel exclusion Chromatography, a method used for separating molecules in a solution based on their size and in sometimes on their molecular weight.
2. In this case we would measure the molecular mass of a protein by comparing the elution volume to mass standards volume.

Calculation of pore size:

1. For chromatography of molecules with molecules with molecular mass near 100 000 the most suitable would be 25 nm pores.