Chapter 19: Q21P (page 488)
What is the advantage of a time-resolved emission measurement with Eu3+versus measurement of fluorescence from organic chromophores?
The main advantage is that they are plagued by background fluorescence from solvent, solutes, and particles.
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Figure 19-6 is a Scatchard plot for the addition of 0-20nM antigen X to a fixed concentration of antibodyPrepare a Scatchard plot from the data in the table and find K for the reaction. The table gives measured concentrations of unbound X and the complex PX. It is recommended that the fraction of saturation should span the range ,-0.2-0.8. What is the range of the fraction of saturation for the data?
This problem can be worked by calculator or with the spreadsheet in Figure 19-4. Consider compounds X and Y in the example labeled “Analysis of a Mixture, Using Equations 19-6” on page 464. Find [X] and [Y] in a solution whose absorbance is 0.233 at 272 nm and 0.200 at 327 nm in a 0.100-cm cell.
Explain how signal amplification is achieved in enzyme linked immunosorbent assays.
Simulating a Job’s plot. Consider the reaction . Suppose that the following mixtures of A and B at a fixed total concentration of
10-4 M are prepared:
(b)Prepare a graph by the method of continuous variation in which you plotversus mole fraction offor each equilibrium constant. Explain the shapes of the curves.
Explain what is done in flow injection analysis and sequential injection. What is the principal difference between the two techniques? Which one is called “lab-on-a-valve”?
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