Chapter 15: Q. 15.6 (page 385)
Discuss the major reasons why molecular phosphorescence spectrometry has not been as widely used as molecular fluorescence spectrometry.
Phosphorescence is time taking, has larger life span and occur at low temperature.
Over 30 million students worldwide already upgrade their learning with Vaia!
All the tools & learning materials you need for study success - in one app.
Get started for free
Why is spectrofluorometry potentially more sensitive than spectrophotometry?
The volumes of a standard solution containing ppm of shown in the table were pipetted into separatory funnels, each containing mL of an unknown zinc solution. Each was extracted with three aliquots of containing an excess of . The extracts were then diluted to mL and their fluorescence measured with a fluorometer. The results were the following:
(a) Construct a working curve from the data.
(b) Calculate a linear least-squares equation for the data.
(c) Calculate the standard deviation of the slope and intercept and the standard deviation about regression.
(d) Calculate the concentration of zinc in the sample.
(e) Calculate a standard deviation for the result in part (d)
Which compound in each of the following pairs would you expect to have a greater fluorescence quantum yield? Explain.
Explain the difference between a fluorescence emission spectrum and a fluorescence excitation spectrum. Which more closely resembles an absorption spectrum?
The following lifetimes were measured for the chloride quenching of quinine sulfate given in Example 15-1. The fluorescence intensities are given in the example.
(a) Plot fluorescence intensity versus [Cl-].
(b) Plot the ratio of intensity to lifetime, F-t versus [Cl-].
(c) Develop a normalization factor to correct the measured fluorescence intensity to that of the solution without quencher.
(d) Plot on the same graph F versus [Cl-] and Fcorr versus [Cl-] .
What do you think about this solution?
We value your feedback to improve our textbook solutions.
