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Chapter 28: Q. 28.16 (page 779)

Describe the differences between single-column and suppressor-column ion chromatography.

Short Answer

Expert verified

Single column ion chromatography combines low capacity columns and conductivity eluents whereas in supressor column ion chromatography a supressor is used.

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01

Step 1. Given information

Describe the differences between single-column and suppressor-column ion chromatography.

02

Differences between single-column and suppressor-column ion chromatography

S. No.Single-column ion chromatographySupressor-column ion chromatography
1In this technique the compounds with low ionic strength are eluted.In this technique the non-ionic compounds are eluted.
2There is no supressor membraneThe supressor membrane is used for the elution of the compounds.
3It is based on the small differences in the conductivity between the eluted and the sample ions.It is based on the ion exchange which has a second exchange column after the original one.
4The analyte ions are nor interfered by the analyte ionic which are seperated on the basis of low capacity ion-exchanger.The analyte ions do not interfere by the non-ionic species which are formed from the conversion of the eluent by ion exchange column.

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Most popular questions from this chapter

What types of species can be separated by HPLC but not by GC?

Assume for simplicity that the HPLC plate height, H, can be given by Equation 26-23 as

H=Bu+CS+CMu=Bu+CuwhereC=CS+CM

(a) By using calculus to find the minimum H, show that the optimal velocity uopt can be expressed as

uopt=BC

(b) Show that this leads to a minimum plate height Hmingiven by

Hmin=2BC

(c) Under some conditions for chromatography, CSis negligible compared to CM. For packed HPLC columns, CM is given by

CM=ฯ‰d2pDM

where ฯ‰ is a dimensionless constant, dpis the particle size of the column packing, and DMis the diffusion coefficient in the mobile phase. The B coefficient can be expressed as

B=2ฮณDM

where ฮณ is also a dimensionless constant. Express uoptand Hminin terms of DM, dp, and the dimensionless constants ฮณand ฯ‰.

(d) If the dimensionless constants are on the order of unity, show that uopt and Hmin can be expressed as

uoptโ‰ˆDMdpandHminโ‰ˆdp

(e)Under the preceding conditions, how could the plate height be reduced by one-third? What would
happen to the optimal velocity under these conditions? What would happen to the number of theoretical platesNfor the same length column?
(f) For the conditions in part (e), how could you maintain the same number of theoretical plates while reducing the plate height by one-third?
(g)The preceding discussion assumes that all band broadening occurs within the column. Name two
sources of extra column band broadening that might also contribute to the overall width of HPLC
peaks.

What is meant by the linear-response range of a detector?

In what way are normal-phase partition chromatography and adsorption chromatography similar?

What is a guard column in partition chromatography?
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