Question

Describe the fundamental difference between ion-exchange and size-exclusion chromatography.

Short answer

The ion exchange is the oldest technique of liquid chromatography used for separation. Whereas, the size-exclusion is the newest technique of the liquid chromatography used for the separation. The ion-exchange separation technique is based on the charge of molecules. And the size-exclusion separation technique is based on the size and shape of molecules.

Step-by-step solution

Step 1. Given information 

Explain the fundamental differences between the size-exclusion and ion-exchange chromatography.

Step 2. Differences between the size-exclusion and ion-exchange chromatography 

S. No.	Ion-exchange chromatography.	Size-exclusion chromatography.
1	This is the oldest technique of liquid chromatography used for separation.	This is the newest technique of liquid chromatography used for the separation.
2	In this technique, the separation is based on the charge of molecules.	In this technique, the separation is based on the size and shape of molecules.
3	In this technique, the interaction takes place between the polar molecules or ions and the sample’s component in the mobile phase.	In this technique, the interaction takes place between the stationary phase and the mobile phase.
4	This technique is used for the analysis of the bio-molecules and charge variant analysis.	This technique is used for the protein mixtures such as trimmers and dimmers etc.