Question

Define (a) retention volume, (b) corrected retention volume, (c) specific retention volume

Short answer

a. Retention volume: volume of eluent.

b. Corrected retention volume:

Step-by-step solution

Part a. Retention volume

Retention volume (V_R) defines the volume of eluent carrier gas. It is the amount of gas that enters the column during chromatography before it leaves the column.

It is calculated using the below mentioned relation-

V_{R =}t_R$\times$F_c

Part b. Corrected retention volume

Corrected retention volume (V_R⁰) refers to the actual volume of eluent in the mobile phase under real conditions in a chromatographic column.

It can be calculated using the following relation-

(V_R⁰) = j $\times$t_{R $\times$}F_c

$\times$

Part c. Specific retention volume

Specific retention volume refers to the net retention volume per gram of stationary phase. It is calculated from the standard chromatographic relation at 273k.