Question

List the variables that lead to (a) band broadening and (b) band separation in GLC.

Short answer

(a) Variables that result in band broadening in chromatography are: very smalland huge flow rates, slow injection rate, film thickness, and packing with large particles.

(b) Variables that cause band separation are: packing with small particles, fast injection of the sample, and thin coating.

Step-by-step solution

band broadening.

$\left(a\right)$Factors that affect broadening in GLC chromatography are:

• very small and huge rates
• slow injection rate
• film's thickness
• packing with large particles.

Band separation.

$\left(b\right)$Factors thatlead to band separation are:

• packing with small particles
• fast injection of the sample
• thin coating