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Why do quantitative and qualitative analyses often require different monochromator slit widths?

Short Answer

Expert verified

Different monochromator slit widths are often investigated in qualitative and quantitative investigations. And for qualitative analysis, identification of a narrow spectral line (absorption or emission) is required.

Step by step solution

01

Step 1. Theory

A monochromater is an optical device that transmits a small band of wavelengths of light or other radiation that may be mechanically selected from a larger range of wavelengths available at the input.

02

Step 2. Explanation

Different monochromator slit widths are often investigated in qualitative and quantitative investigations.

For qualitative analysis, identification of a narrow spectral line (absorption or emission) is required. Quantitative analysis, on the other hand, necessitates a wider slit (absorption or emission) to get a greater signal to noise ratio (higher precision).

As a result, for qualitative analysis, a small slit width is required, whereas for quantitative analysis, a broader slit width is necessary.

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Most popular questions from this chapter

Calculate the absorbance of solutions having twice the percent transmittance of those in Problem 13-2.

Calculate the percent transmittance of solutions having half the absorbance of the solutions in Problem 13-1.

A portable photometer with a linear response to radiation registered 56.3ยตA with the solvent in the light path. The photometer was set to zero with no light striking the detector. Replacement of the solvent with an absorbing solution yielded a response of 36.7ยตA. Calculate

(a) the percent transmittance of the sample solution.

(b) the absorbance of the sample solution.

(c) the transmittance to be expected for a solution in which the concentration of the absorber is one third that of the original sample solution.

(d) the transmittance to be expected for a solution that has twice the concentration of the sample solution.

The following questions concern the relative concentration uncertainty in spectrophotometry.

(a) If the relative concentration uncertainty is given by Equation 13-13, use calculus to show that the minimum uncertainty occurs at 36.8%T. What is the absorbance that minimizes the concentration uncertainty? Assume that sTis independent of concentration.

(b) Under shot-noise-limited conditions, the relative concentration uncertainty is given by Equation 13-14. Another form of the equation for the shot-noise-limited case is 13

scc=-kT-1/2InT

where kis a constant. Use calculus and derive the transmittance and absorbance that minimize the concentration uncertainty.

(c) Describe how you could experimentally determine whether a spectrophotometer was operating under Case I, Case II, or Case III conditions.

A photometer with a linear response to radiation gave a reading of 529mV with the solvent in the light path and 272mV when the solvent was replaced by an absorbing solution. The photometer was set to zero with no light striking the detector. Calculate

(a) the percent transmittance and absorbance of the absorbing solution.

(b) the expected transmittance if the concentration of absorber is one half that of the original solution.

(c) the transmittance to be expected if the light path through the original solution is doubled.

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