Question

The following data were taken from a diode-array spectrophotometer in an experiment to measure the spectrum of the $Co\left(II\right)-EDTA$complex. The column labeled $P_{solution}$is the relative signal obtained with sample solution in the cell after subtraction of the dark signal. The column labeled $P_{solvent}$is the reference signal obtained with only solvent in the cell after subtraction of the dark signal. Find the transmittance at each wavelength, and the absorbance at each wavelength. Plot the spectrum of the compound.

Short answer

The absorbance, transmittance and the plot are given in the final step.

Step-by-step solution

Step 1. Theory

Using data from the $P_{solvent}$signal and the role="math" localid="1645684943339" $P_{solution}$signal, we calculated absorbance and transmittance at each wavelength.

After subtracting the dark signal, $P_{solvent}$is the signal obtained for the solvent in the cell.

After subtracting the dark signal, $P_{solution}$is the signal measured for the sample solution in the cell.

Step 2. Plotting graph

Use formula for transmittance as,

$T=\frac{P_{\text{solution}}\left(\text{signal}\right)}{P_{\text{solvent}}\left(\text{signal}\right)}$

And for absorbance use formula as,

$A={\log }_{10}(\mathrm{T})$

Now on the basis of given values we get the data as,

Now plot the graph,