Chapter 26: Q 26.2. (page 716)
Describe the general elution problem.
When conditions are set for the partition of the late eluting pinnacle, great results for quick eluted pinnacles are not obtained. When settings are specified for early eluting pinnacles, a good result for late eluting pinnacles is not obtained.
Over 30 million students worldwide already upgrade their learning with Vaia!
All the tools & learning materials you need for study success - in one app.
Get started for free
List the variables in chromatography that lead to zone separation
Describe how the retention factor for a solute can be manipulated.
A chromatogram of a two-component mixture on a packed LC column is shown in the following figure. The flow rate was data-custom-editor="chemistry" .
(a) Find the times that components A and B spend in the stationary phase.
(b) Find the retention times for A and B.
(c) Determine the retention factors for the two components.
(d) Find the full widths of each peak and the full width at half-maximum values.
(e) Find the resolution of the two peaks.
(f) Find the average number of plates for the column.
(g) Find the average plate height.
(h) What column length would be needed to achieve a resolution of ?
(i) What time would be required to achieve the resolution in part (h)?
(j) Assume that the column length is fixed at role="math" localid="1649053810406" and the packing material is fixed. What measures could you take to increase the resolution to achieve baseline separation?
(k) Are there any measures you could use to achieve a better separation in a shorter time with the same column as in part (j)?
The relative areas for the five gas chromatographic peaks obtained in the separation of five steroids are given next. Also shown are the relative responses of the detector to the five compounds. Calculate the percentage of each component in the mixture.
The following data are for a liquid chromatographic column
A chromatogram of a mixture of species A, B, C, and D provided the following data:
Calculate
(a) the number of plates from each peak.
(b) the mean and the standard deviation for N.
(c) the plate height for the column.
What do you think about this solution?
We value your feedback to improve our textbook solutions.
