Question

What is gradient elution?

Short answer

Gradient elution is defined as a change in the rate of flow of the mobile phase or a change in the mobile phase's composition.

Step-by-step solution

Step1. Given information

The definition of gradient elution is to be stated.

Step2. Gradient elution

Gradient elution refers to a change in the rate of flow of the mobile phase or a change in the mobile phase's composition. Gradient elution is mostly employed in liquid chromatography to separate two or more analytes in a given sample that is being analyzed.

Gradient elution improves the column's quasi-efficiency. The primary goal of gradient elution is to transfer the retained components as quickly as possible. The compounds that have been retained occupy the uppermost place on the chromatograph's surface.