Question

Write out the steps for the synthesis of each peptide using the Merrifield method:

(a)Ala-Leu-Phe-Phe;

(b) Phe-Gly-Ala-Ile.

Short answer

Merrifield method is a method developed by Bruce Merrifield, and it requires connecting the C-terminus of the peptide chain with a solid in polymeric form. In this method, the product is purified at every step to avoid unwanted cross-reactions.

Step-by-step solution

Merrifield Method

Merrifield method is a method developed by Bruce Merrifield, and it requires connecting the C-terminus of the peptide chain with a solid in polymeric form. In this method, the product is purified at every step to avoid unwanted cross-reactions.

Synthesis of Ala-Leu-Phe-Phe

First of all, make all the Fmoc derivatives for amino acids. The steps for the synthesis of Ala-Leu-Phe-Phe are shown as,

Synthesis of Ala-Leu-Phe-Phe

Synthesis of Phe-Gly-Ala-Ile

Firstly, make all the Fmoc derivatives for amino acids. The steps for the synthesis of Phe-Gly-Ala-Ile are shown as,