Consider the titration of 50.0 mL of 1.0 M glycine hydrochloride [(H3NCH2COOH)Cl], with 1.0 M NaOH.For +H3NCH2COOH, Ka for the carboxylic acid groupis and Kb for the amino group is .
a. Calculate the pH after 25.0 mL, 50.0 mL, and75.0 mL of NaOH has been added. Hint: Refer toSection 8.7 of the text, and treat the titration of+H3NCH2COOH as a diprotic acid titration.
b. Sketch the titration curve and indicate the majoramino acid species present after 0.0 mL, 25.0 mL,50.0 mL, 75.0 mL, and 100.0 mL of 1.0 M NaOHhave reacted completely. Assume the initial pH is1.2 and the pH at the second equivalence pointis 11.7.
c. At what pH do the majority of amino acid molecules have a net charge of zero? This pH is calledthe isoelectric point.
d. At what pH is the net charge of the major aminoacid species present equal to