Calculations for the Purification of a Recombinant Protein The purification of
a recombinant protein is carried out starting with 100 liters of a clarified
cell lysate (i.e., the cells have been lysed, and the cell debris has been
removed to give a clarified solution), which has a total protein concentration
of \(0.36 \mathrm{mg} / \mathrm{ml}\) and a recombinant protein concentration of
\(2.2 \mathrm{U} / \mathrm{ml}\), where U denotes units of biological activity
of the recombinant protein. It is known that the completely pure recombinant
protein has a specific activity of \(40.0 \mathrm{U} / \mathrm{mg}\).
Purification is continued until a chromatography step that yields \(2.0\) liters
of a fraction containing the protein, with a total protein concentration of
\(1.11 \mathrm{mg} / \mathrm{ml}\) and a recombinant protein concentration of
\(43.2 \mathrm{U} / \mathrm{ml}\).For the recombinant protein, calculate the
starting and ending purity, the starting and ending specific activity, and the
percentage yield and fold purification through the chromatography step.