Problem 1
Define transcription.
Problem 2
Explain the difference between the coding strand and the template strand in DNA.
Problem 3
What are the three stages of RNA synthesis?
Problem 4
What components does RNA polymerase require for the synthesis of RNA?
Problem 5
What is the function of the sigma subunit in transcription?
Problem 6
Match each term with its description. (a) Transcription ____________ (b) RNA polymerase _____________ (c) Sigma \((\sigma)\) factor____________ (d) Promoter________________ (e) Consensus sequence_______________ (f) Rho \((\rho)\)______________ (g) Operon________________ (h) Operator_________________ (i) Repressor_______________ (j) Inducer___________ 1\. Recognizes promoter sites 2\. The average order of nucleotides 3\. Repressor binding site 4\. RNA synthesis 5\. DNA sequences that determine the site of transcription initiation 6\. Consists of regulatory elements and proteinencoding genes 7\. In the lac operon, allolactose 8\. Prevents the transcription of structural genes 9\. RNA-synthesis-termination protein 10\. Transcriptional machinery
Problem 7
Compare transcription with replication.
Problem 8
What is a promoter?
Problem 9
Distinguish between a closed promoter complex and an open promoter complex.
Problem 10
The sequence of part of an mRNA is \(5'-AUGGGGAACAGCAAGAGUGGGGCCCUGUCCAAGGAG-3'\),What is the sequence of the DNA coding strand? Of the DNA template strand?
