Problem 1
Define replication, transcription, and translation.
Problem 4
Why is the replication of DNA referred to as a semiconservative process? What is the experimental evidence for the semiconservative nature of the process? What experimental results would you expect if replication of DNA were a conservative process?
Problem 5
What is a replication fork? Why is it important in replication?
Problem 6
Describe the structural features of an origin of replication.
Problem 7
Why is it necessary to unwind the DNA helix in the replication process?
Problem 11
Compare and contrast the properties of the enzymes DNA polymerase I and polymerase III from \(E\). coli
Problem 12
Define processivity, and indicate the importance of this concept in DNA replication.
Problem 16
What would happen to the replication process if the growing DNA chain did not have a free 3 ' end?
Problem 19
Is it unusual that the \(\beta\) -subunits of DNA polymerase III that form a sliding clamp along the DNA do not contain the active site for the polymerization reaction? Explain your answer.
Problem 20
List the substances required for replication of DNA catalyzed by DNA polymerase.
