Ethanol, which is the type of alcohol produced during fermentation, can become toxic. For yeast, it is both a product and a poison.
As the concentration of ethanol increases during fermentation, it can damage the cell membranes and other cellular structures of yeast. This happens because ethanol has a dehydrating effect, drawing out water from cells and disrupting normal cellular functions.
- High ethanol levels can lead to lipid peroxidation, damaging cell membranes.
- It can also denature essential proteins, affecting the yeast's ability to function properly.
Yeast cells have some mechanisms to tolerate ethanol, like altering their membrane compositions to become less permeable to ethanol, but beyond a certain point, these mechanisms fail. Generally, once ethanol concentration reaches a critical level, such as 13%, yeast cells begin to die due to ethanol toxicity.