Question

Transfer \(1 \mathrm{~mL}\) from "AB-Control 3 " to a clean glass test tube. Label it "Control 3."

Short answer

Transfer 1 mL from 'AB-Control 3' to a labeled glass test tube 'Control 3.'

Step-by-step solution

- Gather Materials

Collect a clean glass test tube and a pipette or a micropipette suited for measuring and transferring 1 mL.

- Label the Test Tube

Label the clean glass test tube as 'Control 3' using a lab marker or any other permanent labeling method.

- Measure the Volume

Set the pipette or micropipette to measure 1 mL. If using a pipette, ensure it has a proper tip attached.

- Transfer Solution

Carefully draw 1 mL of the solution from 'AB-Control 3' using the pipette or micropipette. Ensure there are no air bubbles and the volume is accurate.

- Dispense the Solution

Dispense the 1 mL of solution into the labeled 'Control 3' test tube. Ensure all the liquid is transferred properly.

Key concepts

Pipetting Techniques

Pipetting is crucial in laboratory procedures to ensure accurate volume measurement and transfer. Various types of pipettes include manual, electronic, and micropipettes. Micropipettes are often used for small volumes, such as 1 mL, as required in our exercise.
When using a pipette, the technique is significant:

• Avoid bubbles. They can lead to inaccurate measurements.
• Use a steady hand when drawing and dispensing liquids.
• If using a tip, ensure it's securely attached.
Ensure to dispense at eye level, which helps in maintaining accuracy.
Remember to always pre-wet the pipette tip before transferring the actual volume. This helps in achieving consistency.

Labelling Samples

Labeling samples correctly prevents mix-ups and ensures traceability. In our exercise, the test tube is labeled 'Control 3'. Here are some tips for effective labeling:

• Use a permanent marker to avoid smudge.
• Label before adding any solution to prevent label damage.
• Include relevant details like the sample name, date, and your initials if necessary.

Proper labeling ensures that experiments are reproducible and data is reliable.

Volume Measurement

Accurate volume measurement is critical in experiments. Whether using a pipette or a graduated cylinder, always check for your device's calibration.
When setting the pipette to 1 mL, ensure:

• It is properly set to the desired volume.
• The pipette tip is correctly attached.

Hold the pipette at a right angle when drawing up the liquid. Always observe the meniscus—the curve seen at the liquid's surface.
Using the correct technique ensures precision, especially when transferring small volumes.

Solution Transfer

Solution transfer is a fundamental laboratory task. The goal is to transfer liquids without contamination or loss. Follow these steps for effective solution transfer:

• Draw the liquid into the pipette/micropipette slowly to avoid air bubbles.
• Ensure the 1 mL volume is accurate.
• Dispense the solution into the labeled test tube slowly to avoid splashes.

Proper transfer ensures the reliability and accuracy of your experiments.
Ensure the entire contents are transferred to avoid discrepancies in results.