Question

Label the four microtubes containing sterile \(\mathrm{dH}_{2} \mathrm{O}\) \(" \mathrm{~A}, "{ }^{\prime \prime} \mathrm{B}, "{ }^{\prime \prime} \mathrm{C}, "\) and " \(\mathrm{D} . "\)

Short answer

Label the four microtubes with 'A', 'B', 'C', and 'D'.

Step-by-step solution

Title - Gather materials

Gather four sterile microtubes and a marker.

Title - Label the first microtube

Using the marker, write the letter 'A' on the first microtube.

Title - Label the second microtube

Using the marker, write the letter 'B' on the second microtube.

Title - Label the third microtube

Using the marker, write the letter 'C' on the third microtube.

Title - Label the fourth microtube

Using the marker, write the letter 'D' on the fourth microtube.

Key concepts

Labeling Microtubes

Labeling microtubes is a crucial step in any microbiology experiment. Accurate labeling ensures that each sample can be identified correctly and traced later in the experiment. Start by gathering all necessary materials, including microtubes and a permanent marker. It's also essential to prepare a clean and organized workspace to prevent any mix-ups.
Using a permanent marker, label each sterile microtube clearly. Write one letter on each tube in a sequential manner, such as 'A', 'B', 'C', and 'D'. Ensure that the letters are legible and won't easily rub off during handling. Consistent labeling helps maintain the integrity of the samples, especially during multiple transfers and procedures. Proper labeling will save time and reduce errors throughout your experiment.

Sterile Techniques

Sterile techniques are vital to preventing contamination in microbiology labs. Always start with thoroughly washing your hands and wearing appropriate personal protective equipment (PPE), such as gloves, lab coats, and safety goggles. Disinfect the work area with an appropriate antimicrobial solution. This practice helps eliminate any existing microorganisms that could contaminate your samples.
When handling the microtubes, avoid touching the inside or the rims. Use aseptic techniques such as opening the tubes away from airflow and minimizing the time they stay open. Employ tools like sterile pipettes for transferring solutions and make sure to handle these tools only at their handles and not the tips. After completing the procedures, properly dispose of any waste materials and wash your hands again to complete the sterile protocol.

Laboratory Setup

A well-organized laboratory setup is key to efficient and error-free experimental work. Begin by arranging your workspace to include essential equipment and materials within easy reach. Ensure that all surfaces are clean and disinfected before starting the experiment.
Preparation is crucial. Gather all necessary equipment such as sterile microtubes, markers, lab notebooks, and any other specific tools required for the task at hand. Labeling tools and other materials in advance can save time and reduce the chances of errors. Keep track of your experimental procedures in a lab notebook, noting down each step for future reference and reproducibility.
Proper storage of samples is another critical aspect of laboratory setup. Ensure that labeled microtubes and other materials are stored in appropriate conditions, avoiding extreme temperatures or direct sunlight. Maintaining a tidy and organized environment will help you focus on your experiment and minimize the risk of contamination or other issues.