Problem 1
Compare and contrast the following terms: a. CDNA and gene b. RFLP and gene c. DNA probe and gene d. DNA polymerase and DNA ligase e. \(r D N A\) and \(c D N A\) f. genome and proteome
Problem 2
Differentiate the following terms. Which one is "hit and miss"that is, does not add a specific gene to a cell? a. protoplast fusion b. gene gun c. microinjection d. electroporation
Problem 4
Suppose you want multiple copies of a gene you have synthesized. How would you obtain the necessary copies by cloning? By PCR?
Problem 6
Describe an rDNA experiment in two or three sentences. Use the following terms: intron, exon, DNA, mRNA, CDNA, RNA polymerase, reverse transcriptase.
Problem 7
List at least two examples of the use of rDNA in medicine and in agriculture.
Problem 8
You are attempting to insert a gene for saltwater tolerance into a plant by using the Ti plasmid. In addition to the desired gene, you add a gene for tetracycline resistance (tet) to the plasmid. What is the purpose of the tet gene?
Problem 9
How does RNAi "silence" a gene?
Problem 10
This virus family, normally associated with AIDS, may be useful for gene therapy.
