Question

\(\mathrm{PCR}\) a. uses RNA polymerase. b. takes place in huge bioreactors. c. uses DNA replication in vitro. d. makes many nonidentical copies of DNA. e. All of these are correct.

Short answer

The correct statement is: \(c. PCR \) uses DNA replication in vitro.

Step-by-step solution

Analyze statement (a)

PCR uses RNA polymerase. This statement is incorrect. PCR uses DNA polymerase, not RNA polymerase, to replicate DNA.

Analyze statement (b)

PCR takes place in huge bioreactors. This statement is also incorrect. PCR takes place in a small instrument called a thermocycler, which precisely changes temperature during the PCR process.

Analyze statement (c)

PCR uses DNA replication in vitro. This statement is correct. PCR is an in vitro (test tube) method that is used to amplify specific DNA sequences.

Analyze statement (d)

PCR makes many nonidentical copies of DNA. This statement is incorrect. PCR makes many identical copies of a specific DNA sequence, not nonidentical copies.

Analyze statement (e)

All of these are correct. Since not all of the above statements are correct, this statement is incorrect.

Conclusion

Based on our analysis, the correct statement is: c. PCR uses DNA replication in vitro.