Question

The RNA polymerase from bacteriophage T7differs structurally from prokaryotic and eukaryotic RNAPs and is extremely specific for its own promoter. Why do these properties make T7RNAP useful in experiments with recombinant DNA?

Short answer

RNAP has a high level of promoter selectivity because the -terminal is conserved and binds asymmetrically with the promoter.

Step-by-step solution

Definition of RNAP

RNA polymerase, also known as DNA-directed/dependent RNA polymerase (DdRP), is an enzyme that produces RNA from a DNA template and is commonly abbreviated as RNAP or RNApol in molecular biology.

Step 2:  RNAP in experiments with recombinant DNA

The RNA polymerase of T7 bacteriophage is very specific for its promoter due to the presence of cvobnserves C terminal sequence. It allows T7RNAP to transcribe only those genes in recombinant DNA that are associated with its promoters. Hence, it is very useful in recombinant DNA technology.