Chapter 26: Q19P (page 980)
In what ways do histone gene transcripts differ from transcripts of other vertebrate protein-coding genes?
Histone mRNAs are not spliced like those of other protein-coding genes because they lack introns.
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The 5′ end of some bacterial regulatory RNAs are linked to the 3′- OH group of the adenosine residue of NAD+. Propose a biological function for the modification and explain how the activity of the modified RNA might reflect the cell’s overall metabolic activity.
Design an oligonucleotide-based affinity chromatography system for purifying mature mRNAs from eukaryotic cell lysates.
What is the advantage of starting RNA processing before transcription is complete?
Replication of many RNA viruses depends on RNA polymerase. The antiviral drug ribavirin does not inhibit the polymerase but instead increases its error rate. Explain how this affects the virus.
What are the advantages of having multiple types of promoters and enhancers?
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