Chapter 26: Q18P (page 980)
In what ways do histone gene transcripts differ from transcripts of other vertebrate protein-coding genes?
Histone are the basic proteins associated with packaging of DNA in the nucleus of eukaryotes. And undergoes post transcriptional modifications in its N terminal and globular regions.
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Draw a diagram, including exons and introns, of a gene that encodes both membrane-bound and soluble forms of a protein. Explain how one gene can code for two different forms of a protein.
Discuss the advantages, in terms of protein structure and evolution, that result from alternative mRNA splicing?
Explain why inserting 5bp of DNA at the -50position of a eukaryotic gene decreases the rate of RNA polymerase II transcription initiation to a greater extent than inserting 10 bpat the same site.
Human cells contain a protein that binds to the triphosphate groups of RNA. Explain why this protein would be part of the defense against viral infection.
N6 methylation of adenosine residues is one of the most common mRNA modifications. (a) Draw the structure of the modified base. (b) Would you expect the modification to alter the RNA’s ability to form stem-loop structures?
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