Question

Question: Compare DNA polymerase, RNA polymerase, poly(A) polymerase, and CCA-adding polymerase with respect to requirement for a primer, template, and substrates.

Short answer

DNA polymerase needs a primer, template and all four types of dNTP’s as substrates.

RNA polymerase needs a primer, template and all four types of dNTP’s as substrates.

CCA adding polymerase needs primer, template but only ATP and t RNA nucleotidyl transferase as substrates.

Step-by-step solution

 Requirements of different enzymes for their activity

Different types of enzymes have different types of specific requirements for their activity. DNA polymerase, RNA polymerase, poly(A) polymerase, and CCA-adding polymerase have different requirement for a primer, template, and substrates.

Explanation

DNA polymerase needed for the process of replication needs a primer for its extension. This primer can be the small oligonucleotide of pre-mRNA for the poly polymerase. Immature t RNA can be used as an primer for tRNA nucleotidyl transferase enzyme. DNA and RNA strands are used as template by DNA and RNA polymerase, while the DNA polymerase and tRNA nucleotidyl transferase does not require a template.All polymerases require different sets of nucleotides .DNA polymerase uses all four types of deoxyribonucleotides. RNA polymerases uses all four types of ribonucleotides. Poly A uses only ATP and tRNAnucleotidyl transferase uses both ATP and CTP.