Question

(a) In what order would the amino acids Arg, His, and Leu be eluted from a carboxymethyl column at pH6 ?

(b) In what order would Glu, Lys, and Val be eluted from a diethylaminoethyl column at pH 8?

Short answer

a) In the following order Leu, His, Arg would the amino acids Arg, His, and Leu be eluted from a carboxymethyl column at pH 6.

b) In the following order Lys, Val, Glu would Glu, Lys, and Val be eluted from a diethylaminoethyl column at pH 8.

Step-by-step solution

Definition of amino acids

The building blocks of proteins are amino acids, which are the chemical compounds that come together to make proteins. These biomolecules are essential for human growth and development and are engaged in a number of biological and chemical processes in the body.

Find the correct order to be eluted from a carboxymethyl column at pH6.

a) Elution is the process of extracting one material from another using a solvent. Larger and non-polar molecules are the first to elute.

The amino acids Arg, His, and Leu would be eluted from a carboxymethyl column at pH6 in the following order: Leu, His, Arg.

Find the correct order to elute from a diethylaminoethyl column at pH8.

b) Elution is the process of extracting one material from another using a solvent. Larger and non-polar molecules are the first to elute.

Glu, Lys, and Val would be eluted from a diethylaminoethyl column at pH8 in the following order: Lys, Val, Glu.