Chapter 5: Q20P (page 128)
Draw a different phylogenetic tree based on the data in Problem 19.
Short Answer
The required phylogenetic tree is:
Problem presents data in a variety of ways.
Chapter 5: Q20P (page 128)
Draw a different phylogenetic tree based on the data in Problem 19.
The required phylogenetic tree is:
Problem presents data in a variety of ways.
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Get started for freeSeparate cleavage reactions of a polypeptide by CNBr and chymotrypsin yield fragments with the following amino acid sequences. What is the sequence of the intact polypeptide?
CNBr treatment
1. ArgโAlaโTyrโGlyโAsn
2. LeuโPheโMet
3. AspโMet
Chymotrypsin
4. MetโArgโAlaโTyr
5. AspโMetโLeuโPhe
6. GlyโAsn
Protein X has an absorptivity of 0.4mL ยท mg-1ยท cm-1 at 280nm. What is the absorbance at 280nm of a 2.0 mg ยท mL-1solution of protein X? (Assume the light path is 1cm.)
Describe how a protein may be quantified by an assay or by absorbance spectroscopy.
Explain how the number of amino acid differences between homologous proteins can be used to construct a phylogenetic tree.
You are trying to purify a protein that is soluble in a solution of 2 M ammonium sulfate. After centrifugation to remove other proteins that have precipitated at this high salt concentration, you recover the supernatant to assay the target proteinโs activity in a cell culture system.
(a) Explain why the cells die when incubated with the supernatant.
(b) What procedure could you perform to correct the problem? (Hint: See Section 2-1D).
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