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Draw a different phylogenetic tree based on the data in Problem 19.

Short Answer

Expert verified

The required phylogenetic tree is:

Problem presents data in a variety of ways.

Step by step solution

01

Explanation of Concept

A phylogenetic tree, also known as a phylogeny, is a diagram that shows how various species, animals, or genes descended from a common ancestor.

02

Drawing Phylogenetic trees

We compare amino acid sequences of given proteins to create phylogenetic trees. Proteins with more differences have evolved at a faster rate. Proteins and have only one difference, putting them near together on the phylogenetic tree, however Protein has three differences from both, putting it in a different branch.

Therefore, Problem presents data in a variety of ways.

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Most popular questions from this chapter

Electrospray ionization mass spectrometry (ESI-MS) of proteins involves creating positively charged ions of the protein and separating them according to their mass-to-charge ratio (m/z).

(a) What causes the different positive charges on different particles of the protein?

(b) The amino acid composition (in numbers of residues per chain) of hen egg-white lysozyme (HEWL) is as follows:

P 2 Y 3 N 14 H 1

D 7 M 2 L 8 E 2

C 8 R 11 G 12 F 3

A 12 I 6 K 6 V 6

S 10 W 6 T 7 Q 3

What is the maximum positive charge that can be present on a HEWL ion?

Question: Purification tables are often used to keep track of the yield and purification of a protein. The specific activity is a ratio of the amount of the protein of interest, in this case Mb, obtained at a given step (ฮผmol or enzyme units) divided by the amount (mg) of total protein. The yield is the ratio of the amount of the protein of interest obtained at a given step (ฮผmol or enzyme units) divided by the original amount present in the crude extract, often converted to percent yield by multiplying by 100. The fold purification is the ratio of the specific activity of the purified protein to that of the crude preparation.

(a) For the purification table below, calculate the specific activity, % yield, and fold purification for the empty cells.

(b) Which stepโ€”DEAE or affinity chromatographyโ€”causes the greatest loss of Mb?

(c) Which step causes the greater purification of Mb?

(d) If you wanted to use only one purification step, which technique would you choose?

Separate cleavage reactions of a polypeptide by CNBr and chymotrypsin yield fragments with the following amino acid sequences. What is the sequence of the intact polypeptide?

CNBr treatment

1. Argโ€“Alaโ€“Tyrโ€“Glyโ€“Asn

2. Leuโ€“Pheโ€“Met

3. Aspโ€“Met

Chymotrypsin

4. Metโ€“Argโ€“Alaโ€“Tyr

5. Aspโ€“Metโ€“Leuโ€“Phe

6. Glyโ€“Asn

You wish to determine the sequence of a short peptide. Cleavage with trypsin yields three smaller peptides with the sequences Leuโ€“Glu, Glyโ€“Tyrโ€“ Asnโ€“Arg, and Glnโ€“Alaโ€“Pheโ€“Valโ€“Lys. Cleavage with chymotrypsin yields three peptides with the sequences Glnโ€“Alaโ€“Phe, Asnโ€“Argโ€“Leuโ€“Glu, and Valโ€“Lysโ€“Glyโ€“Tyr. What is the sequence of the intact peptide?

Electrospray ionization mass spectrometry (ESI-MS) of proteins involves creating positively charged ions of the protein and separating them according to their mass-to-charge ratio (m/z).

(a) What causes the different positive charges on different particles of the protein?

(b) The amino acid composition (in numbers of residues per chain) of hen egg-white lysozyme (HEWL) is as follows:

P 2 Y 3 N 14 H 1

D 7 M 2 L 8 E 2

C 8 R 11 G 12 F 3

A 12 I 6 K 6 V 6

S 10 W 6 T 7 Q 3

What is the maximum positive charge that can be present on a HEWL ion?

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