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Question: Sketch a phylogenetic tree for the family of homologous proteins

whose partial sequences are given below.

Protein A T L A D K A I S L H D S

Protein B T L G D K A V S I H E S

Protein C T L A D K A I S V H D S

Short Answer

Expert verified

It’s a phylogenetic tree.

Step by step solution

01

Explanation of Concept

A phylogenetic tree, also known as phylogeny, is a diagram that shows how various species, animals, or genes descended from a common ancestor.

02

Sequences of Protein

Except for the amino acid at position 9, the amino acid sequences of proteins and are identical. Protein B differs from Protein and in its sequence.


Therefore, it’s a phylogenetic tree.

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Most popular questions from this chapter

You are using ammonium sulphate to purify protein Q (pI = 5.0) by salting out from a solution at pH 7.0. How should you adjust the pH of the mixture to maximize the amount of protein Q that precipitates?

Consult Table 5-1 to complete the following: (a) On a plot of absorbance at 280 nm versus elution volume, sketch the results of gel filtration of a mixture containing human cytochrome c and bacteriophage T7 RNA polymerase and identify each peak. (b) Sketch the results of SDS-PAGE of the same protein mixture showing the direction of migration and identifying each band.

Explain the origin of orthologous proteins, paralogous proteins, and multidomain proteins.

What fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?

1. 25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn, 5% Gln, 5% Pro

2. 30% Gln, 25% Glu, 20% Lys, 15% Ser, 10% Cys

3. 25% Asn, 20% Gly, 20% Asp, 20% Ser, 10% Lys, 5% Tyr

All three proteins are similar in size and pI, and there is no antibody available for protein 1.

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides:

1. Ala–Val–Cys–Arg–Thr–Gly–Cys–Lys–Asn–Phe–Leu

2. Tyr–Lys–Cys–Phe–Arg–His–Thr–Lys–Cys–Ser

Treatment of the intact polypeptide with trypsin yields fragments with the following amino acid compositions:

3. (Ala, Arg, Cys2, Ser, Val)

4. (Arg, Cys2, Gly, Lys, Thr, Phe)

5. (Asn, Leu, Phe)

6. (His, Lys, Thr)

7. (Lys, Tyr)

Indicate the positions of the disulfide bonds in the intact polypeptide.

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