Chapter 5: Q14CP (page 119)
What types of information can be retrieved from a protein sequence database?
The protein sequence database retrieve protein sequence records by gene name, organism, author name, accession number and a variety of other text terms.
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What fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?
1. 25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn, 5% Gln, 5% Pro
2. 30% Gln, 25% Glu, 20% Lys, 15% Ser, 10% Cys
3. 25% Asn, 20% Gly, 20% Asp, 20% Ser, 10% Lys, 5% Tyr
All three proteins are similar in size and pI, and there is no antibody available for protein 1.
Which peptide has greater absorbance at 280 nm?
A. Gln–Leu–Glu–Phe–Thr–Leu–Asp–Gly–Tyr
B. Ser–Val–Trp–Asp–Phe–Gly–Tyr–Trp–Ala
Explain the origin of orthologous proteins, paralogous proteins, and multidomain proteins.
Explain how the number of amino acid differences between homologous proteins can be used to construct a phylogenetic tree.
You are using ammonium sulphate to purify protein Q (pI = 5.0) by salting out from a solution at pH 7.0. How should you adjust the pH of the mixture to maximize the amount of protein Q that precipitates?
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