Question

What is a DNA library and how can it be screened for a particular gene?

Short answer

The DNA library is a collection of all of an organism's genes. It is very useful in determining the function of a particular gene.

Step-by-step solution

DNA library

A DNA library is a collection of DNA fragments cloned into vectors. Each gene is separated from the genome and replicated in a bacterial cell colony with the same vector DNA as the original.

cDNA library is commonly obtained by converting mRNA to cDNA (complementary DNA). After that, restriction enzymes extract each gene and clone it into a specific bacterial cell.

DNA library screened for particular gene

The cDNA library contains only exons or coding sequences since it comprises cDNA.Thus, the researchers may separate and examine the DNA fragments that interest them.