Question

The recognition sequence for the restriction enzyme Taq1 is T↓CGA. Indicate the products of the reaction of Taq1 with the DNA sequence shown.

$$\begin{gathered} \mathbf{5}\mathbf{\text{'}}\mathbf{‐}\mathbf{ACGTCGAATC}\mathbf{‐}\mathbf{3}\mathbf{\text{'}} \\ \mathbf{3}\mathbf{\text{'}}\mathbf{‐}\mathbf{TGCAGCTTAG}\mathbf{‐}\mathbf{5}\mathbf{\text{'}} \end{gathered}$$

Short answer

When restriction enzyme Taq1 digests the given DNA sequence, the products of the reaction are

$$\begin{gathered} 5\text{'}‐\mathrm{ACGT}+5\text{'}‐\mathrm{CGAATC}‐3\text{'} \\ 3\text{'}‐\mathrm{TGCAGC}‐5\text{'}3\text{'}‐\mathrm{TTAG}‐5\text{'} \end{gathered}$$

Step-by-step solution

Introduction

Restriction enzymes identify and remove nucleotides at or near their unique target sequence on the deoxyribonucleic acid (DNA). Restriction sites or recognition sequenceare terms used to describe these unique sequences.

Action of restriction enzymes on DNA

Sugar-phosphate backbone with nucleotide bases makes up double-stranded DNA. Phosphodiester bonds are cleaved when a restriction enzyme cuts a DNA sequence.

Most of the restriction enzymes perform staggered cuts at or near their specialized recognition point and develop sticky ends.

Taq1

Taq1 is a restriction enzyme that recognizes specific sequence like other restriction enzyme. On each DNA strand, it produces staggered cuts between the'T' and 'C' nucleotides.

As a result, the sugar-phosphate linkages are broken because the restriction enzyme detects and cuts between the 'T' and 'C' bases on each DNA strand. Thus, the products of DNA sequence with the restriction enzyme Taq1 is,

$$\begin{gathered} 5\text{'}‐\mathrm{ACGT}‐3\text{'}+5\text{'}‐\mathrm{CGAATC}‐3\text{'} \\ 3\text{'}‐\mathrm{TGCAGC}‐5\text{'}3\text{'}‐\mathrm{TTAG}‐5\text{'} \end{gathered}$$