Chapter 3: Q14. (page 78)
By how many nucleotides, on average, do the genomes of two Homo sapiens differ?
There is a one-nucleotide difference in the genomes of two Homo sapiens.
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The recognition sequence for the restriction enzyme Taq1 is T↓CGA. Indicate the products of the reaction of Taq1 with the DNA sequence shown.
Kinases are enzymes that transfer a phosphoryl group from a nucleoside triphosphate. Which of the following are valid kinase-catalyzed reactions?
(a)
(b)
A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box).
(a) Since everyone has two copies of each chromosome and therefore two alleles of each gene, what accounts for the appearance of only one allele at some loci?
(b) Which suspect is a possible source of the blood?
(c) Could the suspect be identified using just one of the three STR loci?
(d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?
How many different amino acids could theoretically be encoded by nucleic acids containing four different nucleotides if (a) each nucleotide coded for one amino acid; (b) consecutive sequences of two nucleotides coded for one amino acid; (c) consecutive sequences of three nucleotides coded for one amino acid; (d) consecutive sequences of four nucleotides coded for one amino acid?
Use your answer to Problem 27 to determine the relative pK values of N₃ in cytosine and in uracil
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